P5: EFFECT OF VITAMIN C AND COLD ON GROWTH FACTOR RELEASE FROM PLATELET-RICH PLASMA

Abstract Introduction Platelet-rich plasma (PRP) has gathered great momentum in surgery. The main outcome of the study was to assess whether there a difference growth factor release from PRP with addition vitamin C, cooling, or both C and cooling. Method Blood samples 10 volunteers were centrifuged produce PRP. Activated autologous thrombin produced platelet-poor (PPP), which added activate it. each participant then divided by activation method into three groups: A) cold pack only; B) C) pack/vitamin C. Growth quantification performed for human VEGF, IGF-1 PDGF-AB using enzyme-linked immunosorbent assays. Result At 24 hours, VEGF concentration group significantly higher cf. (p=0.02) cold/vit (p<0.01), whereas IGF but not group. 1 hour PDGF (p<0.01) (p=0.02 p<0.0001 respectively). Conclusion Cooling lead increased at indicating that cooling may sustained factors. There trend treated remain gel-like consistency rather than clot. This prove useful clinical practice where currently activated is constrained topical application only due its consistency. Take-home message factors platelet-rich plasma, provide opportunity injectable